失眠組的條件主要是自身抱怨有失眠問題長達一年以上,受試者依此被區分為失眠組與正常組,而這兩組各再細分為睡眠時數正常(長於六小時) 跟過短(短於六小時),因此有正常組-睡眠時數正常、正常組-睡眠時數短、失眠組-睡眠時數正常與失眠組-睡眠時數短共四組。每位受試者在晚上七點左右將進行五個神經心理功能測驗,包括Mini-Mental State Examination (MMSE)、Symbol Digit Modalities Test (SDMT)、Trail Making Test (TMT)、Benton Visual Retention Test (BVRT)與Thurstone Word Fluency Test (TWFT)。這主要測量的整體認知功能、訊息處理速度、注意力、視覺記憶和語言流暢度。接著受試者會在實驗室睡一晚並進行整晚睡眠紀錄(Polysomngraph, PSG)。
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