在老鼠方面的實驗,他們用選用了兩種因為免疫抑制劑rapamycin而作用的蛋白質,一種稱為mTOR(mammalian Target of Rapamycin)的蛋白質,另一個則是FKBP。他們將mTOR上一個跟FKBP-rapamycin複合物親和性很高的區域──FRB──與螢光酵素的N端部分結合;螢光酵素C端部分則與FKBP結合。結果顯示,只有在rapamycin注入老鼠體內時,螢光酵素片段才會結合而發出螢光,另外在細胞中觀察磷酸化相關的反應也得到很好的效果。
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